OMP ST:1010 ! Escherichia coli K-12 JW0514

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW0514

Synonyms

JW0514-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ppiB737(del)::FRT-kan-FRT

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:922

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +ppiB737(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:28651

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.1mM %/0.5mM
  • normalized fitness= -3.98

OMP_AN:28652

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= -3.65

OMP_AN:28653

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 18 ug/ml
  • normalized fitness= -4.57

OMP_AN:28654

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27505


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Methotrexate hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:165528) Methotrexate hydrate

  • chemical concentration= 25 ug/ml
  • normalized fitness= 4.06

OMP_AN:28655

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:27523


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Minocycline hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:50697) Minocycline hydrochloride

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= 3.5

OMP_AN:28656

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27547


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydrogen peroxide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16240) Hydrogen peroxide

  • chemical concentration= 2 mM
  • normalized fitness= 7.07

OMP_AN:28657

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 3 %
  • normalized fitness= -3.22

OMP_AN:28658

OMP:0007832

increased resistance to sulfonamide

Relative to: OMP_AN:27577


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Sulfamonomethoxine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32164) Sulfamonomethoxine

  • chemical concentration= 100 ug/ml
  • normalized fitness= 3.72

OMP_AN:28659

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27566


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Vancomycin HCl hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9932) Vancomycin HCl hydrate

  • chemical concentration= 50 ug/ml
  • normalized fitness= -7.85

OMP_AN:28660

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27568


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 0.5 mM
  • normalized fitness= -3.97

OMP_AN:28661

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27538


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Oxacillin sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:52134) Oxacillin sodium salt

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.95

OMP_AN:28662

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27551


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Rifampicin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28077) Rifampicin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.81

OMP_AN:28663

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27521


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.1

OMP_AN:28664

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:27524


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tetracycline hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:35006) Tetracycline hydrochloride

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.95

OMP_AN:28665

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27500


Genotype differences:

+lacZ4787(del)( +ppiB737(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Trimethoprim
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924) Trimethoprim

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= -3.46


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page