Minimal medium of Spizizen
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Contents
Purpose
This medium can be used to prepare naturally competent cells of Bacillus subtilis.[1]
Materials Needed
Protocol
- 0.2% ammonium sulfate
- 1.4% dipotassium phosphate
- 0.6% monopotassium phosphate
- 0.1% sodium citrate (2H2O)
- 0.02% magnesium sulfate (7H2O)
- 0.5% glucose
Notes
Papers where this or a similar method has been used
Related Ontology Terms
References
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- ↑ Anagnostopoulos, C & Spizizen, J (1961) REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J. Bacteriol. 81 741-6 PubMed OMPwiki page
