Agarose Gel Electrophoresis

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Purpose

This protocol is used to purify strands of DNA

Materials Needed

-Electrophoresis gel

-Electrophoresis tank

-Power supply

-Buffer

-Loading Dye

-1 kb marker

-Micropipette

-UV light source

Protocol

-Place the hardened gel into the electrophoresis tank with the wells closest to the negative end

-Add enough buffer to cover the gel until the wells are covered

-Load 10 microliters of a 1 kb sample into the first well as a marker using a micropipette

-Mix 10 microliters of the DNA you want to run with .1 microliters of loading dye

-Load the mixture into the second well using a micropipette

-Repeat the above two steps for as many samples as you have

-Attach the nodes from the power supply to the electrophoresis tank

-Set the voltage to the desired level (1-10 V/cm of gel)

-Turn on the power

-When the blue dye from the loading buffer has travelled far enough to allow separation of the DNA fragments, turn off the power

-Use a UV light source to observe the bands

References

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