Category:ECO:0000239 ! chromosome conformation capture sequencing evidence

name: chromosome conformation capture sequencing evidence
def: "A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing." [ECO:MCC]
synonym: "3C sequencing" EXACT []
synonym: "3C-seq" EXACT []
synonym: "4C sequencing" EXACT []
synonym: "4C-seq" EXACT []
synonym: "circularized 3C sequencing" EXACT []
synonym: "circularized 3C-seq" EXACT []
is_a: ECO:0000232 ! chromosome conformation capture-based evidence
comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation.