Category:ECO:0000225 ! chain termination sequencing evidence

name: chain termination sequencing evidence
def: "A nucleotide sequencing assay result derived by the chain termination sequencing method." [ECO:MCC, OBI:0000695]
synonym: "dye terminator sequencing" EXACT []
synonym: "Sanger sequencing" EXACT []
is_a: ECO:0000219 ! nucleotide sequencing assay evidence
comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light.