PMID:6997269

Citation	Wandersman, C , Moreno, F and Schwartz, M (1980) Pleiotropic mutations rendering Escherichia coli K-12 resistant to bacteriophage TP1. J. Bacteriol. 143:1374-83
Abstract	tpo mutations, located at 74 min on the genetic map, rendered Escherichia coli K-12 resistant to TP1, a phage which can use either the OmpF protein or the LamB protein as its receptor. tpo mutants synthesized decreased amounts of OmpF and LamB proteins but increased amounts of the OmpC product, another outer membrane protein. The effect of the tpo mutations in lam B gene expression was transcriptional. It is one facet of the following effect on the maltose regulon: strong decreases in the syntheses of the LamB protein and the periplasmic MalE protein occurred when the regulon was uninduced; a lesser decrease occurred in the syntheses of the LamB protein the MalE protein, and the cytoplasmic MalQ protein (amylomaltase) when the regulon was induced. The tpo mutants were found to be phenotypically identical to the perA mutant recently described by Wanner et al. (J. Bacteriol. 140:229--239, 1979) and to some of the ompB mutants described by Verhoef et al. (Mol. Gen. Genet. 169:137--146, 1979). Mapping and complementation analysis suggested that these three types of mutations belong to the same cistron. Our results bring to at least four the number of clearly distinct phenotypes which can result from mutations at, or close to, ompB, a locus which appears increasingly complex.
Links	PubMed PMC294516
Keywords	Bacterial Proteins/genetics; Coliphages/growth & development; Escherichia coli/genetics; Escherichia coli/physiology; Genes; Maltose/metabolism; Mutation; Operon; Receptors, Virus/genetics; Transcription, Genetic
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1010 NCBI Taxon ID: 83333	Genotype of Reference Strain: tpo Genotype of Experimental Strain : tpo-11	Reference Condition:					Mal - in maltose indicator augar.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1010 NCBI Taxon ID: 83333	Genotype of Reference Strain: tpo Genotype of Experimental Strain : tpo-11	Reference Condition:					Slow growth. Strain has doubling time of 45 min, while WildType was 30 min in complete Medium. Experimental Strain: POP1263 Parental Strain: POP1267
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1010 NCBI Taxon ID: 83333	Genotype of Reference Strain: tpo Genotype of Experimental Strain : tpo-11	Reference Condition:					Slow growth. Strain has doubling time of 120 min, where wild type of 90 min in minimal (M63) glycerol. Experimental Strain: POP1263 Parental Strain: POP1267
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1010 NCBI Taxon ID: 83333	Genotype of Reference Strain: tpo Genotype of Experimental Strain : tpo-11	Reference Condition:					Resistance to TP1 phage. Experimental Strain: POP1263 Parental Strain: POP1010
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1386 NCBI Taxon ID: 83333	Genotype of Reference Strain: top Genotype of Experimental Strain : top-11 in strain POP1387	Reference Condition:					Reduced amounts of ompF protein.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1386 NCBI Taxon ID: 83333	Genotype of Reference Strain: tpo Genotype of Experimental Strain : tpo-22	Reference Condition:					Reduced amount of ompF protein synthesis.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1386 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompB Genotype of Experimental Strain : ompB101	Reference Condition:					Reduced amounts of ompF protein.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: POP1010 NCBI Taxon ID: 83333	Genotype of Reference Strain: tpo Genotype of Experimental Strain : tpo-22 AND tpo-11	Reference Condition: In presence of Maltose Experimental Condition: No Maltose					Resistance to lambda phage.
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Notes

tpo-11 and tpo-22 are envZ

References

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