Phenotype Topics

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  • Cytology
  • Histological Assessment[1]
  • Serological Tests
    • slide agglutination test[15]
    • fluorescent antibody tests[16]
  • Antigenic Characteristics
  • Biochemical Characteristics
    • Cellular Fatty Acid analysis (Bergey's 4-33)
    • catalase test
    • ornithine decarboxylase test
    • oxidase test
    • urease test[17]
    • Voges-Proskauer test
    • Gelatin Liquefaction[18]
  • Prokaryotic Glycosylation
  • Motility [19][20][21]
  • Cell shape/size
    • cocci
    • rod (straight or curved)
    • oval
  • Pathogenic vs. Non-pathogenic
  • Cultural Characteristics[26]
    • Aerobic respiration
    • Anaerobic respiration
    • Thermophile
    • Fermentation
    • Growth on Inorganic Compounds (Chemoauxotrophic)
    • Growth on certain sugars
    • Growth on antibiotics
    • Growth on blood agar
    • Growth on thioglycolate
    • Growth on acetamide agar medium[27]
    • Growth on MacConkey agar[28][29]
    • Growth at certains temperature
  • Morphological Characteristics
    • cocoid body formation
    • cyst formation
    • spore formation
    • pleomorphism
    • mucoidy
  • Replication/Division
    • binary fission
  • Growth characteristics on differential media plates
  • Biofilm Formation[30][31]


References

  1. ↑ Wallis, C et al. (1981) Colorimetric method for rapid determination of bacteriuria. J. Clin. Microbiol. 14 342-6 PubMed OMPwiki page
  2. ↑ CHANCE, HL (1952) Crystal violet as a nuclear stain for gaffkya tetragena and other bacteria. Stain Technol 27 253-8 PubMed OMPwiki page
  3. ↑ LECHTMAN, MD et al. (1965) RAPID METHODS OF STAINING BACTERIAL SPORES AT ROOM TEMPERATURE. J. Bacteriol. 89 848-54 PubMed OMPwiki page
  4. ↑ Wistreich, G & Bartholomew, JW (1969) The binding of crystal violet by isolated bacterial cell-wall material. J. Gen. Microbiol. 59 223-7 PubMed OMPwiki page
  5. ↑ Adams, E (1975) Studies in gram staining. Stain Technol 50 227-31 PubMed OMPwiki page
  6. ↑ Schiff, R et al. (1967) A safranin-fast green stain for the differentiation of the nuclei of rumen protozoa. Stain Technol 42 75-80 PubMed OMPwiki page
  7. ↑ Goren, MB et al. (1978) Some observations of mycobacterial acid-fastness. Am. Rev. Respir. Dis. 118 151-4 PubMed OMPwiki page
  8. ↑ Biswas, BB et al. (1970) Gram staining and its molcecular mechanism. Int. Rev. Cytol. 29 1-27 PubMed OMPwiki page
  9. ↑ Smith, DL & Trentini, WC (1973) On the gram reaction of Caryophanon latum. Can. J. Microbiol. 19 757-60 PubMed OMPwiki page
  10. ↑ Romero, S et al. (1988) Rapid method for the differentiation of gram-positive and gram-negative bacteria on membrane filters. J. Clin. Microbiol. 26 1378-82 PubMed OMPwiki page
  11. ↑ Sizemore, RK et al. (1990) Alternate gram staining technique using a fluorescent lectin. Appl. Environ. Microbiol. 56 2245-7 PubMed OMPwiki page
  12. ↑ Johnson, MJ et al. (1995) Techniques for controlling variability in gram staining of obligate anaerobes. J. Clin. Microbiol. 33 755-8 PubMed OMPwiki page
  13. ↑ Smith, DG (1971) A simple technique for the quantitative assessment of gram positivity. J. Appl. Bacteriol. 34 361-7 PubMed OMPwiki page
  14. ↑ Beveridge, TJ (1990) Mechanism of gram variability in select bacteria. J. Bacteriol. 172 1609-20 PubMed OMPwiki page
  15. ↑ Mayer, ME et al. (1983) Coagglutination for detection and serotyping of bacterial antigens: usefulness in acute pneumonias. Diagn. Microbiol. Infect. Dis. 1 277-85 PubMed OMPwiki page
  16. ↑ Tjiam, KH et al. (1985) Evaluation of the direct fluorescent antibody test for diagnosis of chlamydial infections. Eur. J. Clin. Microbiol. 4 548-52 PubMed OMPwiki page
  17. ↑ Van Horn, KG & Dworkin, BM () Direct gram stain and urease test to detect Helicobacter pylori. Diagn. Microbiol. Infect. Dis. 13 449-52 PubMed OMPwiki page
  18. ↑ Macconkey, A (1906) On the Liquefaction of Gelatin by the Bacillus cloacae. J Hyg (Lond) 6 23-32 PubMed OMPwiki page
  19. ↑ Lonski, J et al. (1977) A quantitative assay to study cell movement in the myxobacteria. J. Cell. Sci. 25 173-8 PubMed OMPwiki page
  20. ↑ Lund, ME et al. (1975) Rapid identification of swarming Proteus using the PathoTec ornithine decarboxylase test strip. J. Clin. Microbiol. 1 478-9 PubMed OMPwiki page
  21. ↑ Bale, MJ et al. (1985) The spot indole test for identification of swarming Proteus. Am. J. Clin. Pathol. 83 87-90 PubMed OMPwiki page
  22. ↑ Palleroni, NJ (1976) Chamber for bacterial chemotaxis experiments. Appl. Environ. Microbiol. 32 729-30 PubMed OMPwiki page
  23. ↑ Aswad, D & Koshland, DE Jr (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. J. Mol. Biol. 97 225-35 PubMed OMPwiki page
  24. ↑ Gentry, MK & Dalrymple, JM (1980) Quantitative microtiter cytotoxicity assay for Shigella toxin. J. Clin. Microbiol. 12 361-6 PubMed OMPwiki page
  25. ↑ Ciapetti, G et al. (1994) Cytotoxicity testing of cyanoacrylates using direct contact assay on cell cultures. Biomaterials 15 63-7 PubMed OMPwiki page
  26. ↑ Robertson, J et al. (1975) Mycoplasma hominis: growth, reproduction, and isolation of small viable cells. J. Bacteriol. 124 1007-18 PubMed OMPwiki page
  27. ↑ Hedberg, M (1969) Acetamide agar medium selective for Pseudomonas aeruginosa. Appl Microbiol 17 481 PubMed OMPwiki page
  28. ↑ 28.0 28.1 Macconkey, A (1905) Lactose-Fermenting Bacteria in Faeces. J Hyg (Lond) 5 333-79 PubMed OMPwiki page
  29. ↑ 29.0 29.1 Macconkey, AT (1908) Bile Salt Media and their advantages in some Bacteriological Examinations. J Hyg (Lond) 8 322-34 PubMed OMPwiki page
  30. ↑ Pedersen, K (1982) Method for studying microbial biofilms in flowing-water systems. Appl. Environ. Microbiol. 43 6-13 PubMed OMPwiki page
  31. ↑ Fessia, SL & Griffin, MJ (1991) A method for assaying biofilm capacity on polyurethane-coated slides. Perit Dial Int 11 144-6 PubMed OMPwiki page
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