Category:ECO:0000079 ! affinity chromatography evidence

name: affinity chromatography evidence
def: "A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound." [ECO:MCC, TAIR:TED]
synonym: "IDA: affinity chromatography" RELATED []
xref: [ MI:0004 "affinity chromatography technology"]
is_a: ECO:0000023 ! affinity evidence
comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand.