Phenotype Topics

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  • Cytology
  • Histological Assessment[1]
    • crystal violet staining[2][3][4][5]
    • safranin staining
    • acid-fastness[6]
    • Gram staining[7][8]
      • Gram negative
      • Gram positive[9]
      • Gram variable
  • Serological Tests
    • slide agglutination test
    • fluorescent antibody tests
  • Antigenic Characteristics
  • Biochemical Characteristics
    • Cellular Fatty Acid analysis (Bergey's 4-33)
    • catalase test
    • oxidase test
    • Voges-Proskauer test
    • Gelatin Liquefaction[10]
  • Prokaryotic Glycosylation
  • Motility [11]
  • Cell shape/size
    • cocci
    • rod (straight or curved)
    • oval
  • Pathogenic vs. Non-pathogenic
  • Cultural Characteristics
    • Aerobic respiration
    • Anaerobic respiration
    • Thermophile
    • Fermentation
    • Growth on Inorganic Compounds (Chemoauxotrophic)
    • Growth on certain sugars
    • Growth on antibiotics
    • Growth on blood agar
    • Growth onthioglycolate
    • Growth on MacConkey agar[15][16]
    • Growth at certains temperature
  • Morphological Characteristics
    • cocoid body formation
    • cyst formation
    • spore formation
    • pleomorphism
    • mucoidy
  • Replication/Division
    • binary fission
  • Growth characteristics on differential media plates
  • Biofilm Formation[17]

References

  1. ↑ Wallis, C et al. (1981) Colorimetric method for rapid determination of bacteriuria. J. Clin. Microbiol. 14 342-6 PubMed OMPwiki page
  2. ↑ CHANCE, HL (1952) Crystal violet as a nuclear stain for gaffkya tetragena and other bacteria. Stain Technol 27 253-8 PubMed OMPwiki page
  3. ↑ LECHTMAN, MD et al. (1965) RAPID METHODS OF STAINING BACTERIAL SPORES AT ROOM TEMPERATURE. J. Bacteriol. 89 848-54 PubMed OMPwiki page
  4. ↑ Wistreich, G & Bartholomew, JW (1969) The binding of crystal violet by isolated bacterial cell-wall material. J. Gen. Microbiol. 59 223-7 PubMed OMPwiki page
  5. ↑ Adams, E (1975) Studies in gram staining. Stain Technol 50 227-31 PubMed OMPwiki page
  6. ↑ Goren, MB et al. (1978) Some observations of mycobacterial acid-fastness. Am. Rev. Respir. Dis. 118 151-4 PubMed OMPwiki page
  7. ↑ Biswas, BB et al. (1970) Gram staining and its molcecular mechanism. Int. Rev. Cytol. 29 1-27 PubMed OMPwiki page
  8. ↑ Smith, DL & Trentini, WC (1973) On the gram reaction of Caryophanon latum. Can. J. Microbiol. 19 757-60 PubMed OMPwiki page
  9. ↑ Smith, DG (1971) A simple technique for the quantitative assessment of gram positivity. J. Appl. Bacteriol. 34 361-7 PubMed OMPwiki page
  10. ↑ Macconkey, A (1906) On the Liquefaction of Gelatin by the Bacillus cloacae. J Hyg (Lond) 6 23-32 PubMed OMPwiki page
  11. ↑ Lonski, J et al. (1977) A quantitative assay to study cell movement in the myxobacteria. J. Cell. Sci. 25 173-8 PubMed OMPwiki page
  12. ↑ Palleroni, NJ (1976) Chamber for bacterial chemotaxis experiments. Appl. Environ. Microbiol. 32 729-30 PubMed OMPwiki page
  13. ↑ Aswad, D & Koshland, DE Jr (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. J. Mol. Biol. 97 225-35 PubMed OMPwiki page
  14. ↑ Gentry, MK & Dalrymple, JM (1980) Quantitative microtiter cytotoxicity assay for Shigella toxin. J. Clin. Microbiol. 12 361-6 PubMed OMPwiki page
  15. ↑ 15.0 15.1 Macconkey, A (1905) Lactose-Fermenting Bacteria in Faeces. J Hyg (Lond) 5 333-79 PubMed OMPwiki page
  16. ↑ 16.0 16.1 Macconkey, AT (1908) Bile Salt Media and their advantages in some Bacteriological Examinations. J Hyg (Lond) 8 322-34 PubMed OMPwiki page
  17. ↑ Pedersen, K (1982) Method for studying microbial biofilms in flowing-water systems. Appl. Environ. Microbiol. 43 6-13 PubMed OMPwiki page
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