PMID:24837471
| Citation |
Lee, JH, Kim, YG, Cho, HS, Ryu, SY, Cho, MH and Lee, J Coumarins reduce biofilm formation and the virulence of Escherichia coli O157:H7. Phytomedicine 21:1037-42 |
|---|---|
| Abstract |
E. coli O157:H7 is the most common cause of hemorrhagic colitis, and no effective therapy exists for E. coli O157:H7 infection. Biofilm formation is closely related to E. coli O157:H7 infection and constitutes a mechanism of antimicrobial resistance. Hence, the antibiofilm or antivirulence approach provides an alternative to antibiotic strategies. Coumarin and its derivatives have a broad range of biological effects, and in this study, the antibiofilm activities of nine coumarins were investigated against E. coli O157:H7. Coumarin or umbelliferone at 50μg/ml was found to inhibit biofilm E. coli O157:H7 formation by more than 80% without affecting bacterial growth. Transcriptional analysis showed that coumarins repressed curli genes and motility genes in E. coli O157:H7, and these findings were in-line with observed reductions in fimbriae production, swarming motility, and biofilm formation. In addition, esculetin repressed Shiga-like toxin gene stx2 in E. coli O157:H7 and attenuated its virulence in vivo in the nematode Caenorhabditis elegans. These findings show that coumarins have potential use in antivirulence strategies against persistent E. coli O157:H7 infection. |
| Links |
PubMed Online version:10.1016/j.phymed.2014.04.008 |
| Keywords |
Animals; Anti-Bacterial Agents/pharmacology; Biofilms/drug effects; Biofilms/growth & development; Caenorhabditis elegans/drug effects; Coumarins/pharmacology; Escherichia coli O157/drug effects; Escherichia coli O157/pathogenicity; Escherichia coli O157/physiology; Escherichia coli O157/ultrastructure; Gene Expression Profiling; Gene Expression Regulation, Bacterial/drug effects; Umbelliferones/pharmacology; Virulence/drug effects |
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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