OMP ST:922 ! Escherichia coli K-12 MS1350

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Revision as of 15:01, 1 July 2016 by Cassandra-wilson (talk | contribs) (Notes)

Strain Summary

Strain Name

Escherichia coli K-12 MS1350

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- lambda- hisG4(Oc) argE3(Oc) thyA galU thiE1 rpsL31(strR) uvrC34 mtl-1 xylA5 rfbC1 mgl-51 kdgK51 e14-(?)

Strain Reference
Strain availability
Ancestry

parent:OMP ST:777 ! Escherichia coli K-12 AB1884

Annotated phenotypes



Notes

Strain MS1350 was prepared by M. Silverman in M. Simon's laboratory from a K-12 strain, AB1884, obtained from J. Adler. The strain was made Lac+, Pro+, Gal+, Thr+,and Leu+ by conjugation with strain KL96, and then Trp- by bromodeoxyuridine auxotroph selection (4). The galU marker was introduced by P1 transduction from W4597 by selecting for Trp+ Gal- recombinants. Thy- strains were obtained by trimethoprim treatment (19). The absence of suppressors was determined by the inability of phage T4 amber B22 to form plaques on this strain.

Phenotype Annotation

Annotation ID not OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes



Additional Information

Links

References

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  1. Komeda, Y et al. (1977) Genetic analysis of Escherichia coli K-12 region I flagellar mutants. J. Bacteriol. 131 801-8 PubMed OMPwiki page
  2. Silverman, M & Simon, M (1973) Genetic analysis of flagellar mutants in Escherichia coli. J. Bacteriol. 113 105-13 PubMed OMPwiki page