Category:ECO:0000231 ! quantitative polymerase chain reaction evidence

name: quantitative polymerase chain reaction evidence
def: "A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample." [ECO:MCC, ECO:SW]
synonym: "Q-PCR evidence" EXACT []
synonym: "qRT-PCR evidence" RELATED []
synonym: "quantitative PCR evidence" EXACT []
synonym: "real-time PCR evidence" EXACT []
synonym: "real-time polymerase chain reaction evidence" EXACT []
is_a: ECO:0000082 ! polymerase chain reaction evidence
comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).