PMID:8820655

Citation	Sandler, SJ (1996) Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12.Mol. Microbiol. 19:871-80
Abstract	The recF and priA genes have roles in DNA repair and homologous recombination. Mutations in these genes also cause decreases in cell viability and alterations in UV-inducible sulAp-lacZ (SOS) expression. To find out if the two genes are in the same or different pathways for viability and SOS expression, the phenotypes of the double mutant strains were studied. The recF priA double mutant showed a lower viability and SOS expression level than either of the single mutants. In the case of cell viability, recF missense mutations decreased viability of a priA2::kan strain two to five-fold whereas recF null priA2::kan double mutants were not viable at all. dnaC809, a mutation that suppresses the UV-sensitive (UVs and Rec- phenotypes of priA2::kan, restored cell viability, but not UV-inducible SOS expression, to a priA recF strain. Since recF is epistatic with recO and recR (recOR) for UV resistance, recOR mutations were also tested with priA2::kan. No overlap was found between recOR and priA for viability and SOS expression. It is concluded that priA and recF have two different overlapping functions in viability and SOS expression that are distinguishable by the effects of dnaC809. The role of recF in a priA2::kan strain in cell viability is a new function for recF and unlike recF's other roles in DNA repair and recombination, is independent of recOR. A new role for priA in UV-inducible SOS expression in a recF mutant is also defined.
Links	PubMed
Keywords	Bacterial Proteins; DNA Repair; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Lac Operon; Mutation; Recombinant Fusion Proteins; Recombination, Genetic; Replication Protein A; SOS Response (Genetics); Ultraviolet Rays
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18536 NCBI Taxon ID: 83333	Genotype of Reference Strain: recF+ Genotype of Experimental Strain : recF349	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	Mutations show approximately 50% decrease in viability relative to the wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: DM4000 recF missense NCBI Taxon ID: 83333	Genotype of Reference Strain: recF+ Genotype of Experimental Strain : recF missense	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	No decrease in cell viability; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: DM4000 priA2::kan recF349 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ Genotype of Experimental Strain : priA2::kan recF349	Reference Condition: 56/2 minimal medium at 37C			ECO:0000052	suppressor/enhancer interaction evidence	abolished cell viability, this, along with both single mutants being able to transduce with the wild type, leads to conclusion that remaining viability in priA2::kan strain due to recF, and remaining viability in redF349 strain due to priA
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18983 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ Genotype of Experimental Strain : priA2::kan	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	Ten-fold decrease in cell viability; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18989 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ Genotype of Experimental Strain : priA2::kan recF143	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	decreased cell viability twofold compared to wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18991 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ Genotype of Experimental Strain : priA2::kan recF4101	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	decreased cell viability fivefold compared to wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18996 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ Genotype of Experimental Strain : priA2::kan recF4115	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	decreased cell viability fivefold compared to wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19028 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recO++ Genotype of Experimental Strain : priA2::kan recO1504::Tn5	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	threefold increase in cell viability; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19029 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recR+ Genotype of Experimental Strain : priA2::kan recR252::Tn10-9	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	slightly increased cell viability; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19002 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ dnaC+ Genotype of Experimental Strain : priA2::kan recF143 dnaC809	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	restored cell viability to that of the wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19111 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ dnaC+ Genotype of Experimental Strain : priA2::kan recF349 dnaC809	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	restored cell viability to that of the wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19109 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ dnaC+ Genotype of Experimental Strain : priA2::kan recF4101 dnaC809	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	restored cell viability to that of the wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18989 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA2::kan recF+ Genotype of Experimental Strain : priA2::kan recF143	Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds	OMP:0005021	decreased basal expression of the SOS response	ECO:0000297	LacZ transcript localization evidence	Decreased relative to priA2::kan single mutant; Reference Figure 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19110 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF+ dnaC+ Genotype of Experimental Strain : priA2::kan recF4115 dnaC809	Reference Condition: 56/2 minimal medium at 37C			ECO:0001027	colony counting evidence	restored cell viability to that of the wild type; Reference Table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC18989 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA+ recF143 Genotype of Experimental Strain : priA2::kan recF143	Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds	OMP:0005020	increased basal expression of the SOS response	ECO:0000297	LacZ transcript localization evidence	Increased relative to recF143 single mutant; Reference Figure 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC19002 NCBI Taxon ID: 83333	Genotype of Reference Strain: priA2::kan recF143 dnaC+ Genotype of Experimental Strain : priAS::kan recF143 dnaC809	Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds	OMP:0005021	decreased basal expression of the SOS response	ECO:0000297	LacZ transcript localization evidence	Decreased relative to priA2::kan recF143 double mutant; Restored basal SOS expression to that of the recF143 mutant strain; Reference Figure 1
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