OMP ST:1293 ! Escherichia coli K-12 JW0212

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Strain Summary

Strain Name

Escherichia coli K-12 JW0212

Synonyms

JW0212-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)::rrnB-3 rph-1 lpcA740(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1151

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +lpcA740(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1152

OMP:0007093

decreased cell motility by swimming

Relative to: OMP_AN:882


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +lpcA740(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:28160

OMP:0007954

decreased population growth at high temperature

Relative to: OMP_AN:27621


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:
  • temperature:45C
  • medium37C
  • medium
  • temperature:45C
  • medium:LB agar
  • medium:High temperature
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 45 C
  • normalized fitness= -4.64

OMP_AN:28161

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27556


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bicyclomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:60584) Bicyclomycin

  • chemical concentration= 10 ug/ml
  • normalized fitness= 3.98

OMP_AN:28162

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27571


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -7.76

OMP_AN:28163

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27514


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 6 uM
  • normalized fitness= 3.33

OMP_AN:28164

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -8.9

OMP_AN:28165

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27572


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 5 uM
  • normalized fitness= 4.4

OMP_AN:28166

OMP:0007173

decreased resistance to host defense peptides

Relative to: OMP_AN:27563


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Indolicidin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (UniProtKB:P33046) Indolicidin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.9

OMP_AN:28167

OMP:0007376

increased resistance to an alkylating agent

Relative to: OMP_AN:27502


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= 3.61

OMP_AN:28168

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27537


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.06 ug/ml
  • normalized fitness= 3.8

OMP_AN:28169

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27570


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.94

OMP_AN:28170

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= 3.71

OMP_AN:28171

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27553


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= 3.6

OMP_AN:28172

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.93

OMP_AN:28173

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -8.92

OMP_AN:28174

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27584


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 1 %
  • normalized fitness= 4.66

OMP_AN:28175

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27588


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -17.48

OMP_AN:28176

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27536


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ceftazidime hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3509) Ceftazidime hydrate

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 3.92

OMP_AN:28177

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27544


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.81

OMP_AN:28178

OMP:0007520

increased population growth in high osmolarity

Relative to: OMP_AN:27595


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 600 mM
  • normalized fitness= 4.14

OMP_AN:28179

OMP:0007010

decreased population growth at alkaline pH

Relative to: OMP_AN:27623


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

pH:8.0?

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
  • pH:8.0
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= pH 8
  • normalized fitness= -4.48

OMP_AN:28180

OMP:0007011

increased population growth at alkaline pH

Relative to: OMP_AN:27623


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:24900259) TAPS

  • chemical concentration= 9
  • normalized fitness= 3.58

OMP_AN:28181

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

pH:4.0?

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
  • pH:4.0
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= pH 4
  • normalized fitness= -11.81

OMP_AN:28182

OMP:0007009

increased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= 4.19

OMP_AN:28183

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27591


Genotype differences:

+lacZ4787(del)( +lpcA740(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= -3.56


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page