OMP ST:71189 ! Escherichia coli K-12 GSO214

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Revision as of 01:54, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 GSO214

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1 ybhU(del)::FRT-kan-FRT

Strain Reference

PMID:19734312[1]

Strain availability
Ancestry

parent:OMP ST:159 ! Escherichia coli K-12 MG1655

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:36959

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= 3.19

OMP_AN:36960

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27561


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bleomycin sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34582) Bleomycin sulfate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -4.56

OMP_AN:36961

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.47

OMP_AN:36962

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27548


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 4 ug/ml
  • normalized fitness= 4.35

OMP_AN:36963

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27509


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cisplatin-cis-Diamineplatinum(II) dichloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27899) Cisplatin-cis-Diamineplatinum(II) dichloride

  • chemical concentration= 100 ug/ml
  • normalized fitness= 5.22

OMP_AN:36964

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.68

OMP_AN:36965

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27501


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 0.8 mM
  • normalized fitness= 4.6

OMP_AN:36966

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27572


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 5 uM
  • normalized fitness= 4.57

OMP_AN:36967

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27515


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fusidic acid sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:126963539) Fusidic acid sodium salt

  • chemical concentration= 5 ug/ml
  • normalized fitness= 3.76

OMP_AN:36968

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -2.99

OMP_AN:36969

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -2.99

OMP_AN:36970

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27512


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydroxyurea
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:44423) Hydroxyurea

  • chemical concentration= 10 mM
  • normalized fitness= 4.45

OMP_AN:36971

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -3.48

OMP_AN:36972

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -3.48


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Hobbs, EC et al. (2010) Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection. J. Bacteriol. 192 59-67 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page