OMP ST:1159 ! Escherichia coli K-12 JW5581

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Strain Summary

Strain Name

Escherichia coli K-12 JW5581

Synonyms

JW5581-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ubiE778(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1075

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +ubiE778(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:36416

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27548

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 2 ug/ml
  • normalized fitness= 4.23

OMP_AN:36417

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27503

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 4.27

OMP_AN:36418

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27584

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 1 %
  • normalized fitness= 4.3

OMP_AN:36419

OMP:0006058

increased resistance to aminoglycoside

Relative to: OMP_AN:27529

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tobramycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28864) Tobramycin

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 4.03

OMP_AN:36420

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27583

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.03 %
  • normalized fitness= 3.55

OMP_AN:36421

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27568

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 0.1 mM
  • normalized fitness= 4

OMP_AN:36422

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27536

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ceftazidime hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3509) Ceftazidime hydrate

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 3.45

OMP_AN:36423

OMP:0007520

increased population growth in high osmolarity

Relative to: OMP_AN:27595

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 150 mM
  • normalized fitness= 3.43

OMP_AN:36424

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27500

Genotype differences:

+lacZ4787(del)( +ubiE778(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Trimethoprim
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924) Trimethoprim

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 3.52
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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