OMP ST:983 ! Escherichia coli K-12 JW5531

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Revision as of 02:40, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW5531

Synonyms

JW5531-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 deaD774(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:896

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +deaD774(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:36236

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -3.38

OMP_AN:36237

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 0.5 ng/ml
  • normalized fitness= -3.55

OMP_AN:36238

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 18 ug/ml
  • normalized fitness= -5.12

OMP_AN:36239

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27520

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= 3.95

OMP_AN:36240

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27545

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Isoniazid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:6030) Isoniazid

  • chemical concentration= 1 mM
  • normalized fitness= -3.74

OMP_AN:36241

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.65

OMP_AN:36242

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.34

OMP_AN:36243

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.34

OMP_AN:36244

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 0.1 mM (LB)
  • normalized fitness= -3.73

OMP_AN:36245

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -5.93

OMP_AN:36246

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27574

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 5 uM
  • normalized fitness= -3.89

OMP_AN:36247

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27564

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phleomycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75044) Phleomycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.88

OMP_AN:36248

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 1 %
  • normalized fitness= -4.62

OMP_AN:36249

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.89

OMP_AN:36250

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27588

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 3.23

OMP_AN:36251

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27539

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.008 ug/ml
  • normalized fitness= 3.85

OMP_AN:36252

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 6 %
  • normalized fitness= -3.18

OMP_AN:36253

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 2.98

OMP_AN:36254

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27608

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 2.98

OMP_AN:36255

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.32

OMP_AN:36256

OMP:0007936

increased resistance to nutrient limitation

Relative to: OMP_AN:27609

Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.32
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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