OMP ST:74219 ! Escherichia coli K-12 JW5290

From OMPwiki
Revision as of 07:34, 23 October 2019 by 127.0.0.1 (talk) (touch)
Jump to navigationJump to search

Strain Summary

Strain Name

Escherichia coli K-12 JW5290

Synonyms

JW5290-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ydjK773(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:35948

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27557


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 15 ug/ml
  • normalized fitness= 4.79

OMP_AN:35949

OMP:0007926

increased anaerobic population growth

Relative to: OMP_AN:27618


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Anaerobic
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • normalized fitness= 4.28

OMP_AN:35950

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27518


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.42

OMP_AN:35951

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= 3.08

OMP_AN:35952

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27548


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.88

OMP_AN:35953

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27501


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 1.2 mM
  • normalized fitness= 3.48

OMP_AN:35954

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27507


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= 4.16

OMP_AN:35955

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.06 ug/ml
  • normalized fitness= -3.2

OMP_AN:35956

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.12

OMP_AN:35957

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.12

OMP_AN:35958

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 10 mM
  • normalized fitness= 3.28

OMP_AN:35959

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27546


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.3

OMP_AN:35960

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27554


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tunicamycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:29699) Tunicamycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.63

OMP_AN:35961

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27566


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Vancomycin HCl hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9932) Vancomycin HCl hydrate

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.98

OMP_AN:35962

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27560


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 300 ug/ml
  • normalized fitness= -3.28

OMP_AN:35963

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27539


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.008 ug/ml
  • normalized fitness= 3.83

OMP_AN:35964

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 0.5 mM
  • normalized fitness= -3.92

OMP_AN:35965

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 4.2

OMP_AN:35966

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 4.2

OMP_AN:35967

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.19

OMP_AN:35968

OMP:0007936

increased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.19

OMP_AN:35969

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27551


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Rifampicin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28077) Rifampicin

  • chemical concentration= 2 ug/ml
  • normalized fitness= -3.61

OMP_AN:35970

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27500


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Trimethoprim
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924) Trimethoprim

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= 4.46

OMP_AN:35971

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27591


Genotype differences:

+lacZ4787(del)( +ydjK773(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= 5.03


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed