OMP ST:74131 ! Escherichia coli K-12 JW5157

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Revision as of 01:28, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW5157

Synonyms

JW5157-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ycfM767(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:35754

OMP:0007954

decreased population growth at high temperature

Relative to: OMP_AN:27621


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:
  • temperature:45C
  • medium37C
  • medium
  • temperature:45C
  • medium:LB agar
  • medium:High temperature
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 45 C
  • normalized fitness= -3.36

OMP_AN:35755

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -6.52

OMP_AN:35756

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= -3.12

OMP_AN:35757

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 1 %
  • normalized fitness= 4.02

OMP_AN:35758

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27553


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 5 ug/ml
  • normalized fitness= -4.81

OMP_AN:35759

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -6.22

OMP_AN:35760

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27588


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.95

OMP_AN:35761

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27568


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= 3.95

OMP_AN:35762

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27589


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin + Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380 + CHEBI:51208) Cefsulodin + Mecillinam

  • chemical concentration= 6 + 0.03 ug/ml
  • normalized fitness= -15.39

OMP_AN:35763

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27539


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.008 ug/ml
  • normalized fitness= -3.43

OMP_AN:35764

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27558


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:D-Cycloserine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:40009) D-Cycloserine

  • chemical concentration= 16 ug/ml
  • normalized fitness= -4.12

OMP_AN:35765

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +ycfM767(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.33


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page