OMP ST:6182 ! Escherichia coli K-12 JW5100

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Revision as of 01:27, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW5100

Synonyms

JW5100-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 tolB789(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1307

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +tolB789(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1308

OMP:0007093

decreased cell motility by swimming

Relative to: OMP_AN:882

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +tolB789(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:35676

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= -4.39

OMP_AN:35677

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.44

OMP_AN:35678

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -7.66

OMP_AN:35679

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27507

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= -13.97

OMP_AN:35680

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.28

OMP_AN:35681

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.28

OMP_AN:35682

OMP:0006061

decreased resistance to tetracycline

Relative to: OMP_AN:27523

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Minocycline hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:50697) Minocycline hydrochloride

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.68

OMP_AN:35683

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.15

OMP_AN:35684

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27584

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 1 %
  • normalized fitness= -4.32

OMP_AN:35685

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27588

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -5.04

OMP_AN:35686

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27583

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.03 %
  • normalized fitness= -4.14

OMP_AN:35687

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27589

Genotype differences:

+lacZ4787(del)( +tolB789(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin + Mecillinam
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380 + CHEBI:51208) Cefsulodin + Mecillinam

  • chemical concentration= 6 + 0.03 ug/ml
  • normalized fitness= -12.4
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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