OMP ST:6129 ! Escherichia coli K-12 JW3714

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Strain Summary

Strain Name

Escherichia coli K-12 JW3714

Synonyms

JW3714-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 atpF765(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1196

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +atpF765(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1197

OMP:0007093

decreased cell motility by swimming

Relative to: OMP_AN:882


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +atpF765(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:34103

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.5mM %/0.5mM
  • normalized fitness= -5.56

OMP_AN:34104

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27557


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 15 ug/ml
  • normalized fitness= -4.04

OMP_AN:34105

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -6.17

OMP_AN:34106

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.94

OMP_AN:34107

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 0.5 ng/ml
  • normalized fitness= -3.58

OMP_AN:34108

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 10 ug/ml
  • normalized fitness= -5.28

OMP_AN:34109

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -3.24

OMP_AN:34110

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27511


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Epinephrine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28918) (-)-Epinephrine

  • chemical concentration= 250 uM
  • normalized fitness= -3.57

OMP_AN:34111

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.03 ug/ml
  • normalized fitness= -3.56

OMP_AN:34112

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.03

OMP_AN:34113

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.03

OMP_AN:34114

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27593


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Norepinephrine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:18357) (-)-Norepinephrine

  • chemical concentration= 1000 uM
  • normalized fitness= -5.06

OMP_AN:34115

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 4 %
  • normalized fitness= -4.23

OMP_AN:34116

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27584


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 1 %
  • normalized fitness= -3.71

OMP_AN:34117

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27516


Genotype differences:

+lacZ4787(del)( +atpF765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Thiolactomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:445629) Thiolactomycin

  • chemical concentration= 50 uM
  • normalized fitness= -4.44


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page