OMP ST:1099 ! Escherichia coli K-12 JW3242

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Revision as of 01:20, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW3242

Synonyms

JW3242-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 aroE721(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1015

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +aroE721(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:33277

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -11.96

OMP_AN:33278

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -11.96

OMP_AN:33279

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 4 ug/ml
  • normalized fitness= -3.56

OMP_AN:33280

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27509

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cisplatin-cis-Diamineplatinum(II) dichloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27899) Cisplatin-cis-Diamineplatinum(II) dichloride

  • chemical concentration= 50 ug/ml
  • normalized fitness= -3.93

OMP_AN:33281

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27555

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin disodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162) Fosfomycin disodium salt

  • chemical concentration= 1 ug/ml
  • normalized fitness= -5.39

OMP_AN:33282

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -11.11

OMP_AN:33283

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -11.11

OMP_AN:33284

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27590

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)

  • chemical concentration= 0.2 ug/ml (in 50 mg/ml G6P)
  • normalized fitness= -3.5

OMP_AN:33285

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -15.19

OMP_AN:33286

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -15.19

OMP_AN:33287

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -7.17

OMP_AN:33288

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -7.17

OMP_AN:33289

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -12.38

OMP_AN:33290

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -12.38

OMP_AN:33291

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -9.11

OMP_AN:33292

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+lacZ4787(del)( +aroE721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -9.11
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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