OMP ST:73297 ! Escherichia coli K-12 JW3169

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Revision as of 00:15, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW3169

Synonyms

JW3169-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 rpoN730(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:33124

OMP:0007959

increased population growth at low temperature

Relative to: OMP_AN:27620

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:
  • temperature:16C
  • medium37C
  • medium
  • temperature:16C
  • medium:LB agar
  • medium:Low temperature
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 16 C
  • normalized fitness= 3.7

OMP_AN:33125

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 0.5 ng/ml
  • normalized fitness= -3.58

OMP_AN:33126

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27592

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Calcofluor White M2R (F3543 Fluorescent Brightener 28)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:50010) Calcofluor White M2R (F3543 Fluorescent Brightener 28)

  • chemical concentration= 50 ug/ml
  • normalized fitness= -3.69

OMP_AN:33127

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 24 ug/ml
  • normalized fitness= -3.72

OMP_AN:33128

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27507

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= 3.17

OMP_AN:33129

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -4.03

OMP_AN:33130

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -4.03

OMP_AN:33131

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +rpoN730(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.39
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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