OMP ST:72859 ! Escherichia coli K-12 JW2491

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Revision as of 00:45, 23 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW2491

Synonyms

JW2491-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 guaA756(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


edit table


Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:31903

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27557

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -10.64

OMP_AN:31904

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -10.03

OMP_AN:31905

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -10.03

OMP_AN:31906

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= -4.27

OMP_AN:31907

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.82

OMP_AN:31908

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27592

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Calcofluor White M2R (F3543 Fluorescent Brightener 28)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:50010) Calcofluor White M2R (F3543 Fluorescent Brightener 28)

  • chemical concentration= 50 ug/ml
  • normalized fitness= -5.97

OMP_AN:31909

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27548

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 6 ug/ml
  • normalized fitness= -3.55

OMP_AN:31910

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.1 %
  • normalized fitness= -10.79

OMP_AN:31911

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -5.35

OMP_AN:31912

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -4.4

OMP_AN:31913

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27511

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Epinephrine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28918) (-)-Epinephrine

  • chemical concentration= 250 uM
  • normalized fitness= -5.45

OMP_AN:31914

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -11.89

OMP_AN:31915

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -11.89

OMP_AN:31916

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -10.69

OMP_AN:31917

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -10.69

OMP_AN:31918

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -10.17

OMP_AN:31919

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -10.17

OMP_AN:31920

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -7.2

OMP_AN:31921

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +guaA756(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -7.2
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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