OMP ST:72850 ! Escherichia coli K-12 JW2479

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Revision as of 23:42, 22 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW2479

Synonyms

JW2479-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 yfgC742(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:31837

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -13.18

OMP_AN:31838

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= -4.74

OMP_AN:31839

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27545

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Isoniazid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:6030) Isoniazid

  • chemical concentration= 1 mM
  • normalized fitness= -4.19

OMP_AN:31840

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.09 ug/ml
  • normalized fitness= -4.78

OMP_AN:31841

OMP:0006061

decreased resistance to tetracycline

Relative to: OMP_AN:27523

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Minocycline hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:50697) Minocycline hydrochloride

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.61

OMP_AN:31842

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27553

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= -4.44

OMP_AN:31843

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -8.04

OMP_AN:31844

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27588

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -13.78

OMP_AN:31845

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27568

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= 3.37

OMP_AN:31846

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27544

Genotype differences:

+lacZ4787(del)( +yfgC742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 2 ug/ml
  • normalized fitness= -3.66
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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