OMP ST:72698 ! Escherichia coli K-12 JW2277

From OMPwiki
Revision as of 23:31, 22 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW2277

Synonyms

JW2277-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 nuoH764(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


edit table


Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:31360

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.5mM %/0.5mM
  • normalized fitness= -10.43

OMP_AN:31361

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -3.1

OMP_AN:31362

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -3.1

OMP_AN:31363

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -11.77

OMP_AN:31364

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.74

OMP_AN:31365

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27571

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -4.1

OMP_AN:31366

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -3.27

OMP_AN:31367

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= -6.47

OMP_AN:31368

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27507

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= -5.13

OMP_AN:31369

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27555

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin disodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162) Fosfomycin disodium salt

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.72

OMP_AN:31370

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27515

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fusidic acid sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:126963539) Fusidic acid sodium salt

  • chemical concentration= 50 ug/ml
  • normalized fitness= -3.46

OMP_AN:31371

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= 3.06

OMP_AN:31372

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= 3.06

OMP_AN:31373

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= -4.81

OMP_AN:31374

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27570

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 20 ug/ml
  • normalized fitness= -3.97

OMP_AN:31375

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -6.05

OMP_AN:31376

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 0.5 %
  • normalized fitness= -5.72

OMP_AN:31377

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27584

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -3.84

OMP_AN:31378

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27567

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Theophylline anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28177) Theophylline anhydrous

  • chemical concentration= 100 ug/ml
  • normalized fitness= -3.77

OMP_AN:31379

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+lacZ4787(del)( +nuoH764(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 4 %
  • normalized fitness= -3.55
edit table


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
Retrieved from "https://microbialphenotypes.org/wiki/index.php?title=OMP_ST:72698_!_Escherichia_coli_K-12_JW2277&oldid=136872"