OMP ST:72026 ! Escherichia coli K-12 JW1284

Strain Summary

Strain Name	Escherichia coli K-12 JW1284
Synonyms	JW1284-1
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 sapD727(del)::FRT-kan-FRT
Strain Reference	PMID:16738554 Coli Genetic Stock Center
Strain availability	Coli Genetic Stock Center
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:30132		OMP:0007895	increased resistance to a surfactant	Relative to: OMP_AN:27581 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Benzalkonium chloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3020) Benzalkonium chloride	chemical concentration= 25 ug/ml normalized fitness= 4.21
OMP_AN:30133		OMP:0007902	increased resistance to bile acids	Relative to: OMP_AN:27586 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Deoxycholate sodium	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9177) Deoxycholate sodium	chemical concentration= 0.1 % normalized fitness= 3.79
OMP_AN:30134		OMP:0007878	increased resistance to an antimicrobial agent	Relative to: OMP_AN:27590 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)	chemical concentration= 0.2 ug/ml (in 50 mg/ml G6P) normalized fitness= 4.18
OMP_AN:30135		OMP:0006063	increased resistance to tetracycline	Relative to: OMP_AN:27523 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Minocycline hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:50697) Minocycline hydrochloride	chemical concentration= 1 ug/ml normalized fitness= 3.36
OMP_AN:30136		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27517 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nigericin sodium salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:16760591) Nigericin sodium salt	chemical concentration= 5 uM normalized fitness= -4.32
OMP_AN:30137		OMP:0006057	decreased resistance to aminoglycoside	Relative to: OMP_AN:27526 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Gentamicin sulfate salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:5312) Gentamicin sulfate salt	chemical concentration= 0.1 ug/ml normalized fitness= -4.32
OMP_AN:30138		OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27598 Genotype differences: +lacZ4787(del)( +sapD727(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron excess-FeSO4 heptahydride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride	chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= 3.14
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References

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