OMP ST:1038 ! Escherichia coli K-12 JW0727

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Revision as of 21:11, 22 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW0727

Synonyms

JW0727-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 tolQ786(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:950

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +tolQ786(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:29147

OMP:0007954

decreased population growth at high temperature

Relative to: OMP_AN:27621

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:
  • temperature:45C
  • medium37C
  • medium
  • temperature:45C
  • medium:LB agar
  • medium:High temperature
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 45 C
  • normalized fitness= -4.72

OMP_AN:29148

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.46

OMP_AN:29149

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 1 %
  • normalized fitness= -16.48

OMP_AN:29150

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27549

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.025 ug/ml
  • normalized fitness= 3.68

OMP_AN:29151

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.75 ug/ml
  • normalized fitness= 3.89

OMP_AN:29152

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -7.24

OMP_AN:29153

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519

Genotype differences:

+lacZ4787(del)( +tolQ786(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 4.87
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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