OMP ST:71673 ! Escherichia coli K-12 JW0724

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Revision as of 21:11, 22 October 2019 by 127.0.0.1 (talk) (touch)

Strain Summary

Strain Name

Escherichia coli K-12 JW0724

Synonyms

JW0724-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ybgT783(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:29116

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.5mM %/0.5mM
  • normalized fitness= -3.24

OMP_AN:29117

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27557

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 2 ug/ml
  • normalized fitness= 3.91

OMP_AN:29118

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.78

OMP_AN:29119

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.86

OMP_AN:29120

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= -8.13

OMP_AN:29121

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27549

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= 3.53

OMP_AN:29122

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 12 ug/ml
  • normalized fitness= -3.67

OMP_AN:29123

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27514

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 3 uM
  • normalized fitness= -4.29

OMP_AN:29124

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27509

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cisplatin-cis-Diamineplatinum(II) dichloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27899) Cisplatin-cis-Diamineplatinum(II) dichloride

  • chemical concentration= 100 ug/ml
  • normalized fitness= 3.79

OMP_AN:29125

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27511

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Epinephrine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28918) (-)-Epinephrine

  • chemical concentration= 1000 uM
  • normalized fitness= 3.64

OMP_AN:29126

OMP:0007377

decreased resistance to an alkylating agent

Relative to: OMP_AN:27502

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= -4.85

OMP_AN:29127

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 3.03

OMP_AN:29128

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 3.03

OMP_AN:29129

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+lacZ4787(del)( +ybgT783(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= 4.7
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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