OMP ST:74710 ! Escherichia coli K-12 CAG41245

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Strain Summary

Strain Name

Escherichia coli K-12 CAG41245

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1 Lamda(rpoHP3::lacZ) lacX74(del) yraP(del)::Cm CmR

Strain Reference

PMID:15968066[1]

Strain availability
Ancestry

parent:OMP ST:74707 ! Escherichia coli K-12 CAG45114

Annotated phenotypes



Notes

In order to confirm that the yraP(del) deletion was responsible for the sensitivity to certain antimicrobial agents, a plasmid carrying the yraP gene was inserted into this strain. The two transformants, CAG41511 and CAG41513, were tested and the EOP experiment was repeated using these transformants. After introducing the plasmids, the transformants displayed the wild-type phenotype.

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:27326

OMP:0007897

abolished resistance to a surfactant

Relative to: OMP_AN:27317


Genotype differences:

+yraP(del) +CmR


Condition differences:

no differences

  • temperature:30C
  • medium:LB broth
  • medium:SDS (3.5%)
ECO:0001165

colony counting evidence used in manual assertion

PMID:15968066[1]

CHEBI:8984 SDS

Table 5; EOP=4E-7

OMP_AN:27327

NOT

OMP:0007216

altered rifamycin resistance

Relative to: OMP_AN:27318


Genotype differences:

+yraP(del) +CmR


Condition differences:

no differences

  • temperature:30C
  • medium:LB broth
  • medium:rifampicin (5 mg/ml)
ECO:0001165

colony counting evidence used in manual assertion

PMID:15968066[1]

CHEBI:28077 rifampicin

Table 5; EOP=0.6

OMP_AN:27328

NOT

OMP:0007134

altered resistance to an antimicrobial agent

Relative to: OMP_AN:27319


Genotype differences:

+yraP(del) +CmR


Condition differences:

no differences

  • temperature:30C
  • medium:LB broth
  • medium:novobiocin (150 mg/ml)
ECO:0001165

colony counting evidence used in manual assertion

PMID:1568066[2]

CHEBI:28368 novobiocin

Table 5; EOP=0.8

OMP_AN:27337

NOT

OMP:0007901

altered resistance to bile acids

Relative to: OMP_AN:27332


Genotype differences:

+yraP(del) +CmR


Condition differences:

no differences

  • temperature:30C
  • medium:MacConkey agar
ECO:0001165

colony counting evidence used in manual assertion

PMID:15968066[1]



Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 1.2 1.3 Onufryk, C et al. (2005) Characterization of six lipoproteins in the sigmaE regulon. J. Bacteriol. 187 4552-61 PubMed OMPwiki page
  2. Vincent, DR et al. (1992) Effect of PCB and DES on rat monoamine oxidase, acetylcholinesterase, testosterone, and estradiol ontogeny. Bull Environ Contam Toxicol 48 884-93 PubMed OMPwiki page