OMP ST:74709 ! Escherichia coli K-12 CAG41254

Strain Summary

Strain Name	Escherichia coli K-12 CAG41254
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	F- Lambda- rph-1 Lamda(rpoHP3::lacZ) lacX74(del) nlpB(del)::Cm CmR
Strain Reference	PMID:15968066
Strain availability
Ancestry	parent:OMP ST:74707 ! Escherichia coli K-12 CAG45114
Annotated phenotypes
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Notes

In order to confirm that the nlpB(del) deletion was responsible for the sensitivity to certain antimicrobial agents, a plasmid carrying the nlpB gene was inserted into this strain. The two transformants, CAG41534 and CAG41536, were tested and the EOP experiment was repeated using these transformants. After introducing the plasmids, the transformants displayed the wild-type phenotype.

Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:27323	NOT	OMP:0007894	altered resistance to a surfactant	Relative to: OMP_AN:27317 Genotype differences: +nlpB(del) +CmR Condition differences: no differences	temperature:30C medium:LB broth medium:SDS (3.5%)	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066	CHEBI:8984 SDS	Table 5; EOP=0.8
OMP_AN:27324		OMP:0007221	abolished resistance to rifamycin	Relative to: OMP_AN:27318 Genotype differences: +nlpB(del) +CmR Condition differences: no differences	temperature:30C medium:LB broth medium:rifampicin (5 mg/ml)	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066	CHEBI:28077 rifampicin	Table 5; EOP=4E-5
OMP_AN:27325	NOT	OMP:0007134	altered resistance to an antimicrobial agent	Relative to: OMP_AN:27319 Genotype differences: +nlpB(del) +CmR Condition differences: no differences	temperature:30C medium:LB broth medium:novobiocin (150 mg/ml)	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066	CHEBI:28368 novobiocin	Table 5; EOP=0.7
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References

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