PMID:19383799

Citation	Malinverni, JC and Silhavy, TJ (2009) An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane. Proc. Natl. Acad. Sci. U.S.A. 106:8009-14
Abstract	The outer membranes (OMs) of gram-negative bacteria have an asymmetric lipid distribution with lipopolysaccharides at the outer leaflet and phospholipids (PLs) at the inner leaflet. This lipid arrangement is essential for the barrier function of the OM and for the viability of most gram-negative bacteria. Cells with OM assembly defects or cells exposed to harsh chemical treatments accumulate PLs in the outer leaflet of the OM and this disrupts lipopolysaccharide organization and increases sensitivity to small toxic molecules. We have identified an ABC transport system in Escherichia coli with predicted import function that serves to prevent PL accumulation in the outer leaflet of the OM. This highly conserved pathway, which we have termed the Mla pathway for its role in preserving OM lipid asymmetry, is composed of at least 6 proteins and contains at least 1 component in each cellular compartment. We propose that the Mla pathway constitutes a bacterial intermembrane PL trafficking system.
Links	PubMed PMC2683108 Online version:10.1073/pnas.0903229106
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Main Points of the Paper

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• ΔmlaC and ΔpldA mutations are synergistic

Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mlaF Genotype of Experimental Strain : mlaF(del)	Reference Condition:					Increase Outer Membrane permeability. Fig 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mlaE Genotype of Experimental Strain : mlaE(del)	Reference Condition:					Increase Outer Membrane permeability. Fig 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mlaD Genotype of Experimental Strain : mlaD(del)	Reference Condition:					Increase Outer Membrane permeability. Fig 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mlaC Genotype of Experimental Strain : mlaC(del)	Reference Condition:					Increase Outer Membrane permeability. Fig 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mlaA Genotype of Experimental Strain : mlaA(del)	Reference Condition:					Increase Outer Membrane permeability. Fig 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mlaC pldA Genotype of Experimental Strain : mlaC(del) pldA(del)	Reference Condition:					Increase Outer Membrane permeability. Fig 1.
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References

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