PMID:783151

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Citation

Ramabhadran, TV (1976) Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation.J. Bacteriol. 127:1587-9

Abstract

Near-ultraviolet radiation (300 to 380 nm) induces a transient growth inhibition in "stringent" (rel+) strains of Escherichia coli, whereas "relaxed" (rel-) strains are only mildly affected. This difference permits rapid isolation of large numbers of relaxed mutants of E. coli.

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Keywords

Bacteriological Techniques; Escherichia coli; Mutation; Ultraviolet Rays

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