PMID:4887874

Citation	Grodzicker, T and Zipser, D (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.J. Mol. Biol. 38:305-14
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Acyltransferases; Chromosome Mapping; Escherichia coli; Genes; Membrane Transport Proteins; Mutation; Operon
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Main Points of the Paper

• π, a new site of initiation of protein synthesis is described
  • operates efficiently to restore permease and acetylase activity to a high level

Materials and Methods Used

• Parent Strain: NG545 (trp-8, Sm^S Hfr)
• Strain: CA77B (Δlac, trp-8, Sm^R F^-)
• Strain: X103B (Δlac pro, trp-8, Sm^R F^-)
• Strain: M107 (Δlac, su_I⁺, Sm^R F^-)
• Strain: ΔLac_W (Δlac, his_-, Sm^R F^-)
• Strain: E29 (lac⁺ , F'/lac⁺, threonine^-, leucine^-, B₁^-, Sm^R)

• Acetylase assay
• β-galactosidase assay

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli	NCBI:562	NG545-pi(1)a	lacZ	able to metabolize melibiose	Growth	growth on melibiose- polar effects	Plating Assay	Table 2
Escherichia coli	NCBI:562	NG545-pi(1)	lacZ		Growth	able to metabolize lactose only in a suI+ background	Plating Assay	Table 2- UAG polar mutation
Escherichia coli	NCBI:562	NG545-pi(1)b	lacZ	unable to metabolize melibiose	Growth	growth on melibiose-polar effects	Plating Assay	Table 2-
Escherichia coli	NCBI:562	NG545-pi(1)a	lacZ	reduced beta-galactosidase activity	Metabolic Activity	trace amounts when induced	Biochemical Assay	Table 2
Escherichia coli	NCBI:562	NG545-pi(1)b	lacZ	reduced beta-galactosidase activity	Metabolic Activity	trace amounts when induced	Biochemical Assay	Table 2
Escherichia coli	NCBI:562	NG545B	lacZ	reduced acetylase activity	Metabolic Activity	4.2% of the wild type level when induced	Biochemical Assay	Tables 3 and 4
Escherichia coli	NCBI:562	NG545-pi(1)aB	lacZ	reduced acetylase activity	Metabolic Activity	35% of the wild type level when induced	Biochemical Assay	Tables 3 and 4
Escherichia coli	NCBI:562	NG545-pi(1)bB	lacZ	reduced acetylase activity	Metabolic Activity	33.5% of the wild type level when induced	Biochemical Assay	Tables 3 and 4
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References

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