PMID:4887874: Difference between revisions

Revision as of 21:43, 15 December 2010

Citation	Grodzicker, T and Zipser, D (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.J. Mol. Biol. 38:305-14
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Acyltransferases; Chromosome Mapping; Escherichia coli; Genes; Membrane Transport Proteins; Mutation; Operon
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π, a new site of initiation of protein synthesis is described
- operates efficiently to restore permease and acetylase activity to a high level

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
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 == Materials and Methods Used ==
-{{LitMaterials}}
+*Parent Strain: NG545 (''trp-8'', Sm<sup>S</sup> Hfr)
+*Strain: CA77B (''Δlac, trp-8'', Sm<sup>R</sup> F<sup>-</sup>)
+*Strain: X103B (''Δlac pro, trp-8'', Sm<sup>R</sup> F<sup>-</sup>)
+*Strain: M107 (''Δlac, su<sub>I</sub><sup>+</sup>'', Sm<sup>R</sup> F<sup>-</sup>)
+*Strain: ΔLac<sub>W</sub> (''Δlac, his<sub>-</sub>'', Sm<sup>R</sup> F<sup>-</sup>)
+*Strain: E29 (''lac<sup>+</sup> '', F'/''lac<sup>+</sup>'', threonine<sup>-</sup>, leucine<sup>-</sup>, B<sub>1</sub><sup>-</sup>, Sm<sup>R</sup>)
+*Acetylase assay
+*β-galactosidase assay
 ==Phenotype Annotations==