Difference between revisions of "Category:ECO:0000118 ! subtractive hybridization evidence"

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'''synonym:''' "IEP: subtractive hybridization" RELATED []<br>
 
'''synonym:''' "IEP: subtractive hybridization" RELATED []<br>
 
'''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br>
 
'''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br>
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'''is_a:''' [[:Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]][[Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]]<br>
 
 
'''comment:''' Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.<br>
 
'''comment:''' Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.<br>
 
}}
 
}}

Revision as of 03:00, 4 March 2018

id: ECO:0000118

name: subtractive hybridization evidence
def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187]
synonym: "IEP: subtractive hybridization" RELATED []
is_a: ECO:0000009 ! transcript expression evidence
comment: Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.

 ECO:0000118

Last version checked
releases/2019-06-14.

14:06:2019 08:25.

rctauber		Last updated
releases/2019-06-14

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