Category:Motility Assay: Difference between revisions
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== Methods == | == Methods == | ||
Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/> | |||
== Examples == | |||
Motility assays were performed by Sperandio et al.<ref name='PMID: 11929534'/> at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h. | |||
== Notes == | == Notes == | ||
== References == | == References == | ||
Revision as of 16:39, 6 June 2011
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Purpose
Technical Details
Materials Needed
Methods
Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. [1]
Examples
Motility assays were performed by Sperandio et al.[2] at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h.
Notes
References
See Help:References for how to manage references in OMPwiki.
- ↑ Rajagopala, SV et al. (2007) The protein network of bacterial motility. Mol. Syst. Biol. 3 128 PubMed
- ↑ Sperandio, V et al. (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol. Microbiol. 43 809-21 PubMed
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