Difference between revisions of "Category:ECO:0000112 ! western blot evidence"
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{{Autobox|1='''id:''' ECO:0000112<br> | {{Autobox|1='''id:''' ECO:0000112<br> | ||
'''name:''' western blot evidence<br> | '''name:''' western blot evidence<br> | ||
− | '''def:''' "A type of | + | '''def:''' "A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique." [ECO:SW, PMID:23050259, TAIR:TED]<br> |
'''synonym:''' "IEP: protein levels (e.g. Western blots)" RELATED []<br> | '''synonym:''' "IEP: protein levels (e.g. Western blots)" RELATED []<br> | ||
'''synonym:''' "protein immunoblot" RELATED []<br> | '''synonym:''' "protein immunoblot" RELATED []<br> | ||
'''synonym:''' "Western blot expression analysis" RELATED []<br> | '''synonym:''' "Western blot expression analysis" RELATED []<br> | ||
− | '''is_a:''' [[:Category:ECO: | + | '''is_a:''' [[:Category:ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence|ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence]][[Category:ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence|ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence]]<br> |
+ | '''is_a:''' [[:Category:ECO:0005593 ! immunodetection assay evidence|ECO:0005593 ! immunodetection assay evidence]][[Category:ECO:0005593 ! immunodetection assay evidence|ECO:0005593 ! immunodetection assay evidence]]<br> | ||
'''comment:''' Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.<br> | '''comment:''' Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.<br> | ||
}} | }} |
Latest revision as of 12:01, 9 August 2016
id: ECO:0000112 name: western blot evidence |
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Pages in category "ECO:0000112 ! western blot evidence"
The following 2 pages are in this category, out of 2 total.