Preparation of Genomic DNA from Bacteria: Difference between revisions
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== Materials Needed == | == Materials Needed == | ||
TE Buffer | |||
10% sodium dodecyl sulfate | |||
20 mg/mL proteinase K | |||
5 mL NaCl | |||
CTAB/NaCl solution | |||
24:1 chloroform/isoamyl alcohol | |||
25:24:1 phenol/chloroform/isoamyl alcohol | |||
Isopropanol | |||
70% ethanol | |||
== Protocol == | == Protocol == | ||
Revision as of 18:33, 14 July 2015
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Purpose
This protocol can be followed to isolate DNA from a given bacteria for further use.
This protocol is based off the protocol found on pages 2.4.1-2.4.2 of Current Protocols in Molecular Biology [1]
Materials Needed
TE Buffer
10% sodium dodecyl sulfate
20 mg/mL proteinase K
5 mL NaCl
CTAB/NaCl solution
24:1 chloroform/isoamyl alcohol
25:24:1 phenol/chloroform/isoamyl alcohol
Isopropanol
70% ethanol
Protocol
Notes
Papers where this or a similar method has been used
<PMIDsummary />
Related Ontology Terms
References
See Help:References for how to manage references in OMPwiki.
- ↑ Ausubel, F. et al. eds. (2002) Current Protocols in Molecular Biology, Wiley
