Southern Blot Analysis: Difference between revisions
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== Materials Needed == | == Materials Needed == | ||
-DNA sample | |||
-0.25 MHCl | |||
-Denaturation Solution (1.5 M NaCl/0.5 M NaOH) | |||
-Neutralization Solution (1.5 M NaCl/0.5 M Tris-Cl) | |||
-20X and 2X SSC | |||
-Sponge (larger than gel to be blotted) | |||
-Whatman 3 MM filter paper sheets | |||
-Nylon membrane | |||
-UV-transparent plastic wrap | |||
-UV transilluminator | |||
== Protocol == | == Protocol == | ||
Revision as of 19:13, 13 July 2015
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Purpose
This protocol is used to immobilize DNA fragments so the DNA can be used for Hybridization Analysis.
Materials Needed
-DNA sample
-0.25 MHCl
-Denaturation Solution (1.5 M NaCl/0.5 M NaOH)
-Neutralization Solution (1.5 M NaCl/0.5 M Tris-Cl)
-20X and 2X SSC
-Sponge (larger than gel to be blotted)
-Whatman 3 MM filter paper sheets
-Nylon membrane
-UV-transparent plastic wrap
-UV transilluminator
Protocol
Notes
Papers where this or a similar method has been used
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Related Ontology Terms
References
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