PMID:2883171: Difference between revisions
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!align=left |Citation | |||
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'''Jenkins, LS and Nunn, WD''' (1987) Regulation of the ato operon by the atoC gene in Escherichia coli.''J. Bacteriol.'' '''169''':2096-102 | |||
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!align=left |Abstract | |||
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The expression of the Ato enzymes, acetyl coenzyme A:acetoacetyl coenzyme A transferase and thiolase II, is required for growth of Escherichia coli on short-chain fatty acids. The structural genes for these enzymes, atoD, atoA, and atoB, respectively, make up the ato operon. A 48-kilodalton protein encoded by atoC was required for the synthesis or activation of the Ato enzymes. The expression of Ato enzyme activities was inducible in atoC+ strains, constitutive in atoCc strains, and noninducible in atoC mutants. Merodiploid studies demonstrated that the atoCc allele is trans-dominant to the atoC+ allele. To study the action of the trans-acting atoC-encoded activator, the promoter of the ato operon was fused to the promoterless galK gene and introduced into a low-copy-number vector. The resulting low-copy-number fusion plasmid was introduced into atoC+, atoC, and atoCc hosts. The expression of the fused galK gene was inducible in the atoC+ host, noninducible in atoC host strains, and constitutive when harbored in the atoCc host. This indicated that the atoC+ and atoCc gene products act at the level of transcription, stimulating the expression of the ato operon. A working model consistent with these results is presented. | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2883171 PubMed] | |||
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!align=left |Keywords | |||
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Acetoacetates; Acetyl-CoA C-Acetyltransferase; Butyrates; Cloning, Molecular; Coenzyme A-Transferases; DNA-Binding Proteins; Enzyme Induction; Escherichia coli; Gene Expression Regulation; Genes, Bacterial; Operon; Promoter Regions, Genetic; Sulfurtransferases | |||
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==Main Points of the Paper == | |||
{{LitSignificance}} | |||
== Materials and Methods Used == | |||
{{LitMaterials}} | |||
==Phenotype Annotations== | |||
{{AnnotationTableHelp}} | |||
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes | |||
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==Notes== | |||
==References== | |||
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Revision as of 18:46, 16 March 2011
| Citation |
Jenkins, LS and Nunn, WD (1987) Regulation of the ato operon by the atoC gene in Escherichia coli.J. Bacteriol. 169:2096-102 |
|---|---|
| Abstract |
The expression of the Ato enzymes, acetyl coenzyme A:acetoacetyl coenzyme A transferase and thiolase II, is required for growth of Escherichia coli on short-chain fatty acids. The structural genes for these enzymes, atoD, atoA, and atoB, respectively, make up the ato operon. A 48-kilodalton protein encoded by atoC was required for the synthesis or activation of the Ato enzymes. The expression of Ato enzyme activities was inducible in atoC+ strains, constitutive in atoCc strains, and noninducible in atoC mutants. Merodiploid studies demonstrated that the atoCc allele is trans-dominant to the atoC+ allele. To study the action of the trans-acting atoC-encoded activator, the promoter of the ato operon was fused to the promoterless galK gene and introduced into a low-copy-number vector. The resulting low-copy-number fusion plasmid was introduced into atoC+, atoC, and atoCc hosts. The expression of the fused galK gene was inducible in the atoC+ host, noninducible in atoC host strains, and constitutive when harbored in the atoCc host. This indicated that the atoC+ and atoCc gene products act at the level of transcription, stimulating the expression of the ato operon. A working model consistent with these results is presented. |
| Links | |
| Keywords |
Acetoacetates; Acetyl-CoA C-Acetyltransferase; Butyrates; Cloning, Molecular; Coenzyme A-Transferases; DNA-Binding Proteins; Enzyme Induction; Escherichia coli; Gene Expression Regulation; Genes, Bacterial; Operon; Promoter Regions, Genetic; Sulfurtransferases |
| edit table |
Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
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Notes
References
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