Difference between revisions of "PMID:20727918"
(Table edited by Tkveton via TableEdit) |
(Table edited by JimHu via TableEdit) |
||
| Line 75: | Line 75: | ||
Reference figure 2; | Reference figure 2; | ||
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli; | NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli; | ||
| − | phenotype is unchanged | + | phenotype is unchanged. |
| | | | ||
| Line 1,034: | Line 1,034: | ||
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup>'' | *Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup>'' | ||
| − | *Genotype of Experimental Strain: | + | *Genotype of Experimental Strain: ‘’SOS-R1 NfsA uvrB(del)’’ |
| | | | ||
| − | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) | + | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) |
| | | | ||
OMP:0005024 | OMP:0005024 | ||
| Line 1,063: | Line 1,063: | ||
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup>'' | *Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup>'' | ||
| − | *Genotype of Experimental Strain: | + | *Genotype of Experimental Strain: ‘’SOS-R1 NfsA uvrB(del)’’ |
| | | | ||
| − | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) | + | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) |
| | | | ||
OMP:0005024 | OMP:0005024 | ||
| Line 1,092: | Line 1,092: | ||
*Genotype of Reference Strain: ''SOS-R1 NfsB uvrB<sup>+</sup>'' | *Genotype of Reference Strain: ''SOS-R1 NfsB uvrB<sup>+</sup>'' | ||
| − | *Genotype of Experimental Strain: | + | *Genotype of Experimental Strain: ‘’SOS-R1 NfsB uvrB(del)’’ |
| | | | ||
| − | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) | + | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) |
| | | | ||
OMP:0005024 | OMP:0005024 | ||
| Line 1,121: | Line 1,121: | ||
*Genotype of Reference Strain: ''SOS-R1 NemA uvrB<sup>+</sup>'' | *Genotype of Reference Strain: ''SOS-R1 NemA uvrB<sup>+</sup>'' | ||
| − | *Genotype of Experimental Strain: | + | *Genotype of Experimental Strain: ‘’SOS-R1 NemA uvrB(del)’’ |
| | | | ||
| − | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) | + | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) |
| | | | ||
OMP:0005024 | OMP:0005024 | ||
| Line 1,150: | Line 1,150: | ||
*Genotype of Reference Strain: ''SOS-R1 YfkO uvrB<sup>+</sup>'' | *Genotype of Reference Strain: ''SOS-R1 YfkO uvrB<sup>+</sup>'' | ||
| − | *Genotype of Experimental Strain: | + | *Genotype of Experimental Strain: ‘’SOS-R1 YfkO uvrB(del)’’ |
| | | | ||
| − | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) | + | *Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar) |
| | | | ||
OMP:0005024 | OMP:0005024 | ||
| Line 1,163: | Line 1,163: | ||
| | | | ||
Reference figure 3; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis | Reference figure 3; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| | | | ||
Revision as of 12:59, 26 February 2015
| Citation |
Prosser, GA, Patterson, AV and Ackerley, DF (2010) uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.J. Biotechnol. 150:190-4 |
|---|---|
| Abstract |
CB1954 is an anti-cancer prodrug that can be reduced at either of two nitro groups to form cytotoxic metabolites. We describe here two efficient and previously uncharacterized nitroreductases, YfkO from Bacillus subtilis which reduces CB1954 exclusively at the 4-NO(2) position, and NfsA from Klebsiella pneumoniae which preferentially reduces the 2-NO(2) group. Utilizing these novel enzymes, together with three previously characterized nitroreductases, we demonstrate that the Escherichia coli SOS-chromotest assay can differentially detect the 4-nitro versus 2-nitro reduction products of CB1954 following deletion of the nucleotide excision repair gene uvrB, but not mismatch repair (mutS) or methyltransferase (ada/ogt) genes. These findings may hold significance for identification and selection of nitroreductases for CB1954-mediated gene therapy, particularly when targeting tumors that are deficient in nucleotide excision repair. Moreover, we demonstrate that comparative SOS chromotest analysis in wild type and uvrB mutant strains can be used to determine whether or not nucleotide excision repair plays a significant role in processing DNA damage resulting from activation of different nitroaromatic prodrugs. |
| Links |
PubMed Online version:10.1016/j.jbiotec.2010.08.007 |
| Keywords |
Antineoplastic Agents; Aziridines; Bacillus subtilis; Bacterial Proteins; DNA Helicases; Escherichia coli Proteins; Gene Deletion; Kinetics; Klebsiella pneumoniae; Metronidazole; Molecular Conformation; Molecular Sequence Data; Nitrofurazone; Nitroreductases; SOS Response (Genetics) |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli; phenotype is unchanged. |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005023 |
presence of an SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis; phenotype is unchanged |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; NemA= 4-NO2 nitroreductase only |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 3; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 3; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 3; NfsB= 2-NO2 or 4-NO2 nitroreductase |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 3; NemA= 4-NO2 nitroreductase only |
|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0005024 |
increased induced expression of the SOS response |
ECO:0000297 |
LacZ transcript localization evidence |
Reference figure 3; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis |
|
| edit table |
Notes
References
See Help:References for how to manage references in OMPwiki.
