PMID:137233

Citation	Deutch, CE, Scarpulla, RC, Sonnenblick, EB and Soffer, RL (1977) Pleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase. J. Bacteriol. 129:544-6
Abstract	A mutant of Escherichia coli that lacks leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase had diminished activities of L-phenylalanyl-transfer ribonucleic acid synthetase and tryptophanase, grew faster than its parent with aspartic acid as the sole nitrogen source, accumulated higher levels of enterochelin in the medium during iron limitation, and exhibited an abnormal morphology.
Links	PubMed PMC234957
Keywords	Acyltransferases/metabolism; Aspartic Acid/metabolism; Enterobactin/metabolism; Escherichia coli/cytology; Escherichia coli/enzymology; Escherichia coli/metabolism; Mutation; Phenylalanine-tRNA Ligase/metabolism; Tryptophanase/metabolism
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: W4977 NCBI Taxon ID: 83333	Genotype of Reference Strain: aat Genotype of Experimental Strain : aat-1	Reference Condition:	OMP:0000073	cell shape phenotype			Abnormal Morphology appearing as clubs, spheres or ellipsoids.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: W4977 NCBI Taxon ID: 83333	Genotype of Reference Strain: aat Genotype of Experimental Strain : aat-1	Reference Condition: Aspartic Acid as Sole Nitrogen Source Experimental Condition: Aspartic Acid as sole Nitrogen Source	OMP:0000282	increased growth rate			In the presence of Aspartic Acid as the sole nitrogen source, the mutants doubling time increase by twice.
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Notes

• OMP Cell shape: What exists implies a state, thus there is no relative phenotypes. Must create phenotypes for all shapes keeping in mind that some colonies will have a mixed and oddly shaped mutants that might not fall into predefined shapes we create.

• ECO term: Observation of cell shape. Simple and microscope?

References

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