Difference between revisions of "Category:ECO:0000223 ! 454 pyrosequencing evidence"
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{{Autobox|1='''id:''' ECO:0000223<br> | {{Autobox|1='''id:''' ECO:0000223<br> | ||
'''name:''' 454 pyrosequencing evidence<br> | '''name:''' 454 pyrosequencing evidence<br> | ||
| − | '''def:''' "A high throughput nucleotide sequencing | + | '''def:''' "A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand." [EFO:0005016]<br> |
'''synonym:''' "pyrosequencing" BROAD [OBI:0000730]<br> | '''synonym:''' "pyrosequencing" BROAD [OBI:0000730]<br> | ||
'''is_a:''' [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]<br> | '''is_a:''' [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]<br> | ||
| − | '''comment:''' 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.<br> | + | '''comment:''' 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.\n\nThe target strand is usually prepared by fragmentation, adaptor ligation, and amplification by emulsion PCR.<br> |
}} | }} | ||
== Usage Notes == | == Usage Notes == | ||
Latest revision as of 03:00, 4 December 2017
| id: ECO:0000223 name: 454 pyrosequencing evidence |
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