Difference between revisions of "Category:ECO:0000118 ! subtractive hybridization evidence"
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{{Autobox|1='''id:''' ECO:0000118<br> | {{Autobox|1='''id:''' ECO:0000118<br> | ||
'''name:''' subtractive hybridization evidence<br> | '''name:''' subtractive hybridization evidence<br> | ||
| − | '''def:''' " | + | '''def:''' "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187]<br> |
'''synonym:''' "IEP: subtractive hybridization" RELATED []<br> | '''synonym:''' "IEP: subtractive hybridization" RELATED []<br> | ||
'''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br> | '''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br> | ||
'''is_a:''' [[:Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]][[Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]]<br> | '''is_a:''' [[:Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]][[Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]]<br> | ||
| + | '''comment:''' Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.<br> | ||
}} | }} | ||
== Usage Notes == | == Usage Notes == | ||
Revision as of 03:00, 4 December 2017
| id: ECO:0000118 name: subtractive hybridization evidence |
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