Difference between revisions of "Category:ECO:0000226 ! chromatin immunoprecipitation evidence"

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(update ECO:0000226_!_chromatin_immunoprecipitation_evidence page)
(update ECO:0000226_!_chromatin_immunoprecipitation_evidence page)
 
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'''xref:''' [ MI:0402 "chromatin immunoprecipitation assay"]<br>
 
'''xref:''' [ MI:0402 "chromatin immunoprecipitation assay"]<br>
 
'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]]<br>
 
'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]]<br>
 +
'''relationship:''' IAO:0000136: [http://gowiki.tamu.edu/index.php/Category:GO:0043565_!_is_about_sequence-specific_DNA_binding GO:0043565 ! is about sequence-specific DNA binding]<br>
 +
'''relationship:''' OBI:0000312: OBI:0001956 ! is_specified_output_of assay using chromatin immunoprecipitation<br>
 
'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>
 
'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>
 
}}
 
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Latest revision as of 02:01, 16 June 2018

id: ECO:0000226

name: chromatin immunoprecipitation evidence
def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]
synonym: "ChIP evidence" EXACT []
xref: [ MI:0402 "chromatin immunoprecipitation assay"]
is_a: ECO:0000085 ! immunoprecipitation evidence
relationship: IAO:0000136: GO:0043565 ! is about sequence-specific DNA binding
relationship: OBI:0000312: OBI:0001956 ! is_specified_output_of assay using chromatin immunoprecipitation
comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).

 ECO:0000226

Last version checked
releases/2019-06-14.

14:06:2019 08:25.

rctauber		Last updated
releases/2019-06-14

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