OMP ST:947 ! DH1 miniFparC10: Difference between revisions

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Table edited by Kcunningham via TableEdit
Kcunningham (talk | contribs)
3,011 edits
Table edited by Kcunningham via TableEdit
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*temperature:42C
*temperature:42C
*medium:Kanamycin (
*medium:Kanamycin
*medium:L agar
*medium:L agar
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OMP_AN:726
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OMP:0007391
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abolished viability
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Relative to: OMP_AN:725
<hr>Genotype differences:<br>
no differences
<hr>Condition differences:<br>
medium:IPTG (100 uM);KanamycinKanamycin (
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*temperature:42C
*medium:IPTG (100 uM)
*medium:Kanamycin
*medium:L agar
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ECO:0000181
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in vitro assay evidence
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PMID:9077455
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CHEBI:6104 Kanamycin
CHEBI:61448 IPTG
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DNA nicking activity instead of transesterification


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Revision as of 21:16, 12 July 2016

Strain Summary

Strain Name

DH1 miniFparC10

Synonyms

parC10 mutant

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype
Strain Reference

PMID:9077455[1]

Strain availability
Ancestry

parent:DH1 miniFparC+

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID not OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:725

OMP:0007392

viable cell

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:42C
  • medium:Kanamycin
  • medium:L agar
 ECO:0000181

in vitro assay evidence

PMID:9077455[1]

CHEBI:6104 Kanamycin

OMP_AN:726

OMP:0007391

abolished viability

Relative to: OMP_AN:725

Genotype differences:

no differences

Condition differences:

medium:IPTG (100 uM);KanamycinKanamycin (

  • temperature:42C
  • medium:IPTG (100 uM)
  • medium:Kanamycin
  • medium:L agar
 ECO:0000181

in vitro assay evidence

PMID:9077455[1]

CHEBI:6104 Kanamycin CHEBI:61448 IPTG

DNA nicking activity instead of transesterification

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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 1.2 Yokochi, T et al. (1996) DNA nicking by Escherichia coli topoisomerase IV with a substitution mutation from tyrosine to histidine at the active site. Genes Cells 1 1069-75 PubMed
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