Difference between revisions of "Antimicrobial Peptide Extraction"
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===Occidiofungin extraction=== | ===Occidiofungin extraction=== | ||
| + | -Culture the ''Burkholderia contaminans'' bacteria in Potato Dextrose Broth at 25 C for at least 5 days | ||
| + | |||
| + | -Centrifuge the bacteria at 13,000 rpm for 20 minutes | ||
| + | |||
| + | -Pour the supernatant into 2000 mL glass flask with a stir bar and add 0.5 volume of Sodium Thiosulfate (i.e. 750 mL of supernatant, 375 grams Sodium Thiosulfate) | ||
| + | |||
| + | -Stir the mixture for 2 hours | ||
| + | |||
| + | -Centrifuge the mixture at 13,000 rpm for 20 minutes | ||
| + | |||
| + | -Discard the supernatant and let the protein precipitate dry overnight | ||
| + | |||
| + | -Wash the protein out of the centrifuge tubes with 35% Acetonitrile into a plastic tube | ||
===Mutacin 1140 extraction=== | ===Mutacin 1140 extraction=== | ||
Revision as of 12:06, 14 July 2015
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Contents
Purpose
Materials Needed
Protocol
Occidiofungin extraction
-Culture the Burkholderia contaminans bacteria in Potato Dextrose Broth at 25 C for at least 5 days
-Centrifuge the bacteria at 13,000 rpm for 20 minutes
-Pour the supernatant into 2000 mL glass flask with a stir bar and add 0.5 volume of Sodium Thiosulfate (i.e. 750 mL of supernatant, 375 grams Sodium Thiosulfate)
-Stir the mixture for 2 hours
-Centrifuge the mixture at 13,000 rpm for 20 minutes
-Discard the supernatant and let the protein precipitate dry overnight
-Wash the protein out of the centrifuge tubes with 35% Acetonitrile into a plastic tube
Mutacin 1140 extraction
Notes
Papers where this or a similar method has been used
Related Ontology Terms
References
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