Antimicrobial Peptide Extraction: Difference between revisions
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===Occidiofungin extraction=== | ===Occidiofungin extraction=== | ||
-Culture the ''Burkholderia contaminans'' bacteria in Potato Dextrose Broth at 25 C for at least 5 days | |||
-Centrifuge the bacteria at 13,000 rpm for 20 minutes | |||
-Pour the supernatant into 2000 mL glass flask with a stir bar and add 0.5 volume of Sodium Thiosulfate (i.e. 750 mL of supernatant, 375 grams Sodium Thiosulfate) | |||
-Stir the mixture for 2 hours | |||
-Centrifuge the mixture at 13,000 rpm for 20 minutes | |||
-Discard the supernatant and let the protein precipitate dry overnight | |||
-Wash the protein out of the centrifuge tubes with 35% Acetonitrile into a plastic tube | |||
===Mutacin 1140 extraction=== | ===Mutacin 1140 extraction=== | ||
Revision as of 18:06, 14 July 2015
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Purpose
Materials Needed
Protocol
Occidiofungin extraction
-Culture the Burkholderia contaminans bacteria in Potato Dextrose Broth at 25 C for at least 5 days
-Centrifuge the bacteria at 13,000 rpm for 20 minutes
-Pour the supernatant into 2000 mL glass flask with a stir bar and add 0.5 volume of Sodium Thiosulfate (i.e. 750 mL of supernatant, 375 grams Sodium Thiosulfate)
-Stir the mixture for 2 hours
-Centrifuge the mixture at 13,000 rpm for 20 minutes
-Discard the supernatant and let the protein precipitate dry overnight
-Wash the protein out of the centrifuge tubes with 35% Acetonitrile into a plastic tube
Mutacin 1140 extraction
Notes
Papers where this or a similar method has been used
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Related Ontology Terms
References
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