PMID:4599365

Citation	Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium
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Main Points of the Paper

• amber mutations in dnaA were isolated

Materials and Methods Used

• Integrative suppression
• EtBr sensitivity assay
• Monitor growth rate
• Thymidine incorporation to measure DNA synthesis rate

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
		CRT-46		streptomycin resistant	Resistance to		Plating Assay
		CRT-46		abolished lactose utilization	Metabolic Activity		Plating Assay
		CRT-46		threonine auxotroph	Growth		Plating Assay
		CRT-46		abolished lactose utilization	Metabolic Activity		Plating Assay
		CRT-46		leucine auxotroph	Growth		Plating Assay
		CRT-46		isoleucine auxotroph	Growth		Plating Assay
		CRT-46		thiamine auxotroph	Growth		Plating Assay
		CRT-46		thymine auxotroph	Growth		Plating Assay
Escherichia coli		NS475	dnaA311(Am)	temperature sensitive defect in initiation of DNA replication	Metabolic Activity	thymidine incorporation	Biochemical Assay	Figure 4A
Escherichia coli		Hfr-120/6		thiamine auxotroph	Growth		Plating Assay
Escherichia coli		Hfr-120/6		thymine auxotroph	Growth		Plating Assay
Escherichia coli		PC2		thymine auxotroph	Growth		Plating Assay
Escherichia coli		PC2		leucine auxotroph	Growth		Plating Assay
Escherichia coli		PC2		streptomyin resistant
Escherichia coli	NCBI:	Hfr-120/6		temperature sensitive defect in initiation of DNA replication
Escherichia coli		NS476	dnaA366(Am)	temperature sensitive defect in initiation of DNA replication	Metabolic Activity	thymidine incorporation	Biochemical Assay	Figure 4B
Escherichia coli		NS477	dnaA91(Am)	temperature sensitive defect in initiation of DNA replication	Metabolic Activity	thymidine incorporation	Biochemical Assay	Figure 4C
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References

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