Category:ECO:0000025 ! hybrid interaction evidence: Difference between revisions

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'''name:''' hybrid interaction evidence<br>
'''name:''' hybrid interaction evidence<br>
'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>
'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>
'''xref:''' [http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-MI:MI\:0090 "protein complementation assay"]<br>
'''xref:''' [ MI:0090 "protein complementation assay"]<br>
'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence|ECO:0000023 ! affinity evidence]]<br>
'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence|ECO:0000023 ! affinity evidence]]<br>
'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>
'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>

Latest revision as of 22:59, 24 October 2017

id: ECO:0000025

name: hybrid interaction evidence
def: "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]
xref: [ MI:0090 "protein complementation assay"]
is_a: ECO:0000023 ! affinity evidence
comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.
Last version checked
releases/2019-06-14.

14:06:2019 08:25.

rctauber		Last updated
releases/2018-09-14

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